Phase I – Immunization
You have the choixe between Dromedary and Lama. The dromedary provides more sequence variability and better stability.
Standard immunization (day 1/21/42/63/73) or Rapid immunization (day 1/7/14/21/28).
Phase II – Lymphocyte isolation, cDNA preparation, Amplification and phage library
cDNA is generated via RNA isolation, amplified via PCR, and a diverse phage library is generated for screening against the antigen.